NDLI: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Please wait, while we are loading the content...

Production of a therapeutic protein by fusing it with two fragments of the carboxyl-terminal peptide of human chorionic gonadotropin β-subunit in Pichia pastoris

Recombinant production of a shell matrix protein in Escherichia coli and its application to the biomimetic synthesis of spherulitic calcite crystals

Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)-octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells

Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae

Highly enantioselective production of a chiral intermediate of sitagliptin by a novel isolate of Pseudomonas pseudoalcaligenes

Algicidal effect of hybrid peptides as potential inhibitors of harmful algal blooms

Expression, purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari

Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase

Purification and characteristics of a novel bacteriocin produced by Enterococcus faecalis L11 isolated from Chinese traditional fermented cucumber

Simultaneous Cr(VI) reduction and Zn(II) biosorption by Stenotrophomonas sp. and constitutive expression of related genes

Combined effects of engineered tendon matrix and GDF-6 on bone marrow mesenchymal stem cell-based tendon regeneration

Lentivirus-mediated transfection of chondroitinase ABC gene without the bacterial leader sequence enables long-term secretion of functional chondroitinase ABC in human bone marrow stromal cells

Biotechnology Letters : Volume 38, Issue 4, April 2016

Biotechnology Letters : Volume 38, Issue 3, March 2016

Biotechnology Letters : Volume 38, Issue 2, February 2016

Biotechnology Letters : Volume 38, Issue 1, January 2016

Biotechnology Letters : Volume 37

Biotechnology Letters : Volume 36

Biotechnology Letters : Volume 35

Biotechnology Letters : Volume 34

Biotechnology Letters : Volume 33

Biotechnology Letters : Volume 32

Biotechnology Letters : Volume 31

Biotechnology Letters : Volume 30

Biotechnology Letters : Volume 29

Biotechnology Letters : Volume 28

Biotechnology Letters : Volume 27

Biotechnology Letters : Volume 26

Biotechnology Letters : Volume 25

Biotechnology Letters : Volume 24

Biotechnology Letters : Volume 23

Biotechnology Letters : Volume 22

Biotechnology Letters : Volume 21

Biotechnology Letters : Volume 20

Biotechnology Letters : Volume 19

Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Content Provider	Springer Nature Link
Author	Woon, James Sy Keen Mackeen, Mukram Mohamed Sudin, Amirul Husna bin Mahadi, r Muhammad Illias, Rosli Md Murad, Abdul Munir Abdul Bakar, Farah Diba Abu
Copyright Year	2016
Abstract	To express and determine the hydrolytic activity of a cellobiohydrolase (TTCBH6B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris. Ttcbh6B encodes a protein of 507 amino acid residues with a predicted molecular mass of 54 kDa. TTCBH6B contains a familial 6-glycosyl hydrolase catalytic domain and a type I carbohydrate-binding module. TTCBH6B was expressed and purified to homogeneity but the purified enzyme was inactive against Avicel. It could, however, digest Celluclast-treated Avicel producing cellobiose (0.27 μmol min−1 mg−1). To determine the substrate preferences of TTCBH6B, oligosaccharides of varying numbers of subunits were generated by acid hydrolysis of Avicel and fluorescently tagged. Peaks corresponding to oligosaccharides containing three to six glucose units were reduced to cellobiose after addition of TTCBH6B.TTCBH6B is active against shorter oligosaccharides rather than polysaccharides.
Starting Page	825
Ending Page	832
Page Count	8
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	5
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-02-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	2-Aminobenzamide Avicel Carbohydrate-binding module Cellobiose Celluclast Endoglucanases Glycosyl hydrolases Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris.

The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable β-1,4-xylanase with exo- and endo-activity

Purification, characterization and modular organization of a cellulose-binding protein, CBP105, a processive β-1,4-endoglucanase from Cellulomonas flavigena

Quantitative analysis of oligosaccharide structure of glycoproteins

A low molecular mass cutinase of Thielavia terrestris efficiently hydrolyzes poly(esters)

A family 5 β-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic enzyme features

Cloning, heterologous expression, and characterization of Thielavia terrestris glucoamylase

Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Similar Documents

Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris.

The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable β-1,4-xylanase with exo- and endo-activity

Purification, characterization and modular organization of a cellulose-binding protein, CBP105, a processive β-1,4-endoglucanase from Cellulomonas flavigena

Quantitative analysis of oligosaccharide structure of glycoproteins

A low molecular mass cutinase of Thielavia terrestris efficiently hydrolyzes poly(esters)

A family 5 β-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic enzyme features

Cloning, heterologous expression, and characterization of Thielavia terrestris glucoamylase

Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris