NDLI: Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized cells of recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia

Content Provider	Springer Nature Link
Author	Oh, Hye Jin Shin, Kyung Cheol Oh, Deok Kun
Copyright Year	2013
Abstract	Recombinant Escherichia coli, expressing the oleate hydratase gene of Stenotrophomonas maltophilia, was permeabilized by sequential treatments with 0.125 M NaCl and 2 mM EDTA. The optimal conditions for the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized cells were 35 °C and pH 7.0 with 0.1 % (v/v) Tween 40, 50 g permeabilized cells l−1, and 17.5 g α-linolenic acid l−1. Under these conditions, permeabilized cells produced 14.3 g 10-hydroxy-12,15(Z,Z)-octadecadienoic acid l−1 after 18 h, with a conversion yield of 82 % (g/g) and a volumetric productivity of 0.79 g l−1 h−1. These values were 17 and 168 % higher than those obtained by nonpermeabilized cells, respectively. The concentration, yield, and productivity of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid obtained by permeabilized cells are the highest reported thus far.
Starting Page	1487
Ending Page	1493
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	35
Issue Number	9
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-05-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell permeabilization 10-Hydroxy-12,15(Z,Z)-octadecadienoic acid α-Linolenic acid Oleate hydratase Stenotrophomonas maltophilia Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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