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| Content Provider | Springer Nature Link |
|---|---|
| Author | Gao, Xiao Rong Wang, Guo Kun Su, Qiao Wang, Yan An, Li Jia |
| Copyright Year | 2007 |
| Abstract | A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T3 transgenic seeds as compared to 64 FTU/kg in wild-type plants. |
| Starting Page | 1781 |
| Ending Page | 1787 |
| Page Count | 7 |
| File Format | |
| ISSN | 01415492 |
| Journal | Biotechnology Letters |
| Volume Number | 29 |
| Issue Number | 11 |
| e-ISSN | 15736776 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2007-07-04 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Pollen tube pathway transformation Linear DNA Phytase gene Transgenic soybean Biochemistry Applied Microbiology Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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