NDLI: Acidic pH conditions induce dissociation of the haem from the protein and destabilise the catalase isolated from Aspergillus terreus

Content Provider	Springer Nature Link
Author	Vatsyayan, Preety Goswami, Pranab
Copyright Year	2010
Abstract	The stability (half-life, t½) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t½ ~8.5 months at pH ≤ 6) versus alkaline conditions (t½ ~15 months at pH 8–12). Acidic conditions induce the dissociation of haem from CAT, as revealed from a reduction in the Soret peak intensity at 405 nm and an increase in the peak current at Fe3+/Fe2+ redox potentials. This increase in current is attributed to the facile electron transfer from the free haem generated on the electrode surface as a result of its disintegration from the insulating protein matrix. The haem isolated from CAT at acidic condition was reconstituted with apo-CAT at alkaline denaturing conditions to regenerate the CAT activity.
Starting Page	347
Ending Page	351
Page Count	5
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	33
Issue Number	2
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-10-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aspergillus terreus Catalase Stability Haem Reconstitution Biochemistry Applied Microbiology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Acidic pH conditions induce dissociation of the haem from the protein and destabilise the catalase isolated from Aspergillus terreus