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| Content Provider | Springer Nature Link |
|---|---|
| Author | Rashid, Muhammad Hamid Javed, Muhammad Rizwan Kawaguchi, Takashi Sumitani, Jun ichi Arai, Motoo |
| Copyright Year | 2008 |
| Abstract | FI-Carboxymethylcellulase (cmc1; family 12) is one of the endoglucanases of Aspergillus aculeatus and consists of single polypeptide chain of 221 amino acids. The cmc1 gene was expressed in Aspergillus oryzae niaD300 (niaD−) under promoter 8142. The plasmid pCMG14 carrying the cmc1 gene at PstI site was used as a source of the gene (920 bp) and Aspergillus oryzae was successfully transformed by the plasmid pNAN-cmc1 (harboring cmc1 gene). The plasmid was integrated in Aspergillus oryzae niaD300 genome at niaD locus and the transformed fungus constitutively produced very high amounts of endoglucanases when grown on glucose, maltose, soluble starch and wheat bran. |
| Starting Page | 2165 |
| Ending Page | 2172 |
| Page Count | 8 |
| File Format | |
| ISSN | 01415492 |
| Journal | Biotechnology Letters |
| Volume Number | 30 |
| Issue Number | 12 |
| e-ISSN | 15736776 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2008-07-26 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aspergillus aculeatus Aspergillus oryzae Endoglucanase Expression cloning Biochemistry Applied Microbiology Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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