NDLI: Production of β-carotene by expressing a heterologous multifunctional carotene synthase in Yarrowia lipolytica

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Production of β-carotene by expressing a heterologous multifunctional carotene synthase in Yarrowia lipolytica

Content Provider	Springer Nature Link
Author	Gao, Shuliang Tong, Yangyang Zhu, Li Ge, Mei Jiang, Yu Chen, Daijie Yang, Sheng
Copyright Year	2017
Abstract	To obtain functional expression of a heterologous multifunctional carotene synthase containing phytoene synthase, phytoene dehydrogenase, and lycopene β-cyclase activities encoded by carS from Schizochytrium sp. in order to allow Yarrowia lipolytica to produce β-carotene.To increase the integration efficiency of a 3.8 kb carS under the control of P GPD promoter with a 2 kb selection marker, ura3, along with a geranylgeranyl diphosphate synthase (GGS1) expression cassette (~10 kb in total), was inserted into the Y. lipolytica chromosome, and the DNA assembler method was combined with double chromosomal deletions of ku70 and ku80. This method resulted in a 13.4-fold increase in integration efficiency compared with the original method, reaching 63% (10/16). The resulting recombinant Y. lipolytica produced 0.41 mg β-carotene per g dry cell weight, while the wild type did not produce any indicating the functionality of the multifunctional carotene synthase in Y. lipolytica.Expression of GGS1 and a multifunctional carotene synthase from Schizochytrium sp. in Y. lipolytica led to β-carotene production. DNA assembler efficiency was greatly increased by the deletion of ku70 and ku80, which resulted in decreased in vivo nonhomologous end-joining (NHEJ) in Y. lipolytica.
Starting Page	921
Ending Page	927
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	39
Issue Number	6
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-03-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	β-Carotene Carotene synthase complex carS DNA assembler Multifunctional carotene synthase Yarrowia lipolytica Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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