NDLI: Sus scrofa matrix attachment region enhances expression of the PiggyBac system transfected into HEK293T cells

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Sus scrofa matrix attachment region enhances expression of the PiggyBac system transfected into HEK293T cells

Content Provider	Springer Nature Link
Author	Wang, Xin Jian Wang, Jiang Wang, Yue Ying Guo, Yu Jie Chu, Bei Bei Yang, Guo Yu
Copyright Year	2016
Abstract	To determine the effects of the Sus scrofa matrix attachment region (SusMAR) on transgene expression in HEK293T cells.Three expression vectors with the MAR at different sites in the PiggyBac (PB) transposon vector backbone were compared: two MARs flanking the β-galactosidase (β-gal) expression cassette, and one at the upstream or downstream site. Bos taurus MAR (BosMAR) and a β-gal expression cassette without MARs were the positive and negative controls, respectively. Compared to the control, β-gal activity of all SusMAR and BosMAR vectors was significantly improved in the presence of PB transposase (PBase). However, only the downstream SusMAR and upstream BosMAR vectors showed increased expression in the absence of PBase. Expression was significantly increased in all vectors with the PBase group compared to those without the PBase group. Gene copy numbers were not increased compared to the negative control.SusMAR enhanced recombinant gene expression levels and stability in HEK293T cells, was not increase transgene copy number. These results could facilitate the development of vectors for stable production of therapeutic proteins.
Starting Page	949
Ending Page	958
Page Count	10
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	6
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Matrix attachment region PiggyBac transposon Therapeutic protein production Transgene copy number Transgene expression Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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