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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhu, Hai Ying Jin, Long Guo, Qing Luo, Zhao Bo Li, Xiao Chen Zhang, Yu Chen Xing, Xiao Xu Xuan, Mei Fu Zhang, Guang Lei Luo, Qi Rong Wang, Jun Xia Cui, Cheng Du Li, Wen Xue Cui, Zheng Yun Yin, Xi Jun Kang, Jin Dan |
| Copyright Year | 2017 |
| Abstract | To investigate the effect of the small molecule, RepSox, on the expression of developmentally important genes and the pre-implantation development of rhesus monkey–pig interspecies somatic cell nuclear transfer (iSCNT) embryos.Rhesus monkey cells expressing the monomeric red fluorescent protein 1 which have a normal (42) chromosome complement, were used as donor cells to generate iSCNT embryos. RepSox increased the expression levels of the pluripotency-related genes, Oct4 and Nanog (p < 0.05), but not of Sox2 compared with untreated embryos at the 2–4-cell stage. Expression of the anti-apoptotic gene, Bcl2, and the pro-apoptotic gene Bax was also affected at the 2–4-cell stage. RepSox treatment also increased the immunostaining intensity of Oct4 at the blastocyst stage (p < 0.05). Although the blastocyst developmental rate was higher in the group treated with 25 µM RepSox for 24 h than in the untreated control group (2.4 vs. 1.2%, p > 0.05), this was not significant.RepSox can improve the developmental potential of rhesus monkey–pig iSCNT embryos by regulating the expression of pluripotency-related genes. |
| Starting Page | 775 |
| Ending Page | 783 |
| Page Count | 9 |
| File Format | |
| ISSN | 01415492 |
| Journal | Biotechnology Letters |
| Volume Number | 39 |
| Issue Number | 5 |
| e-ISSN | 15736776 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2017-02-28 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Embryos (monkey–pig) Interspecies (monkey–pig) somatic cell nuclear transfer mRFP1 Pluripotency RepSox Rhesus monkey Microbiology Biotechnology Applied Microbiology Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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