NDLI: Construction of a Bacterial Artificial Chromosome Library and Characterization of hrp/hrc Gene Cluster of Pseudomonas Syringae Pathovar tagetis LMG5090

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Construction of a Bacterial Artificial Chromosome Library and Characterization of hrp/hrc Gene Cluster of Pseudomonas Syringae Pathovar tagetis LMG5090

Content Provider	Springer Nature Link
Author	Song, Eun Sung Park, Young Jin Chae, Soo Cheon Kim, Jeong Gu Cho, Hee Jung Lee, Gil Bok Lee, Byoung Moo
Copyright Year	2006
Abstract	Pseudomonas syringae pv. tagetis causes apical chlorosis of several plant species in the Asteraceae, including marigold. As a means to facilitate the isolation of pathogenicity genes and to characterize the genome of this bacterium, we have constructed a bacterial artificial chromosome library of P. syringae pv. tagetis strain LMG5090. The library consists of 1,536 clones with insert size ranging from 30 to 160 kb and an average size of 86 kb. Based upon colony hybridization, the BAC clone 420E23 containing the hrp/hrc gene cluster encoding the type III secretion system was identified from this library and subsequently shotgun sequenced. The hrp/hrc gene cluster of P. syringae pv. tagetis has a 23 kb sequence which contains 27 open reading frames. Comparative analysis of the hrp/hrc gene cluster of P. syringae pv. tagetis LMG5090, P. syringae pv. tomato DC3000, P. syringae pv. syringae B728a, and P. syringae pv. phaseolicola 1448A revealed that the entire hrp/hrc gene cluster of P. syringae pv. tagetis is conserved and identically arranged in all four pathovars
Starting Page	969
Ending Page	977
Page Count	9
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	28
Issue Number	13
e-ISSN	15736776
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-06-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BAC library DNA sequencing hrp Gene cluster Pseudomonas syringae pv. tagetis Type III secretion system Microbiology Biochemistry Applied Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Construction of a Bacterial Artificial Chromosome Library and Characterization of hrp/hrc Gene Cluster of Pseudomonas Syringae Pathovar tagetis LMG5090

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