NDLI: Genome shuffling to generate recombinant yeasts for tolerance to inhibitors present in lignocellulosic hydrolysates

Content Provider	Springer Nature Link
Author	Cheng, Clint Almario, Maria P. Kao, Katy C.
Copyright Year	2015
Abstract	To investigate the use of genome shuffling to generate recombinants from previously generated hydrolysates-tolerant strains to improve tolerance of Saccharomyces cerevisiae to one or more inhibitory by-products present in lignocellulosic hydrolysates.Recombinants of previously evolved strains of S. cerevisiae were generated and analyzed for their relative performance in the individual inhibitors furfural, acetic acid, 5-(hydroxymethyl)-furfural (HMF) and in synthetic hydrolysates. One recombinant exhibited a 100 % fitness increase in the presence of HMF as compared to the wild-type diploid, while another stain exhibited a 13 % fitness increase in the presence of furfural. Furthermore, for one of these recombinants, these increases in fitness were specific to the inhibitor HMF and to synthetic hydrolysates rather than being due to a general increase in fitness. Mutations present in the evolved hydrolysates-tolerant mutants were identified via whole-genome resequencing.Recombinants of S. cerevisiae were produced with increased tolerance to inhibitory by-products present in hydrolysates of lignocellulosic biomass and identified potential genetic determinants associated with this phenotype.
Starting Page	2193
Ending Page	2200
Page Count	8
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	37
Issue Number	11
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-06-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acetic acid Furfural Genome shuffling 5-(Hydroxymethyl)furfural Lignocellulosic biomass Saccharomyces cerevisiae Yeast Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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