NDLI: Preparation of a purified, inactivated Japanese encephalitis (JE) virus vaccine in Vero cells

Content Provider	Springer Nature Link
Author	Pyo Hong, Sun Yoo, Wang Don Putnak, Robert Srivastava, Ashok K. Eckels, Kenneth H. Chung, Yong Ju Mo Rho, Hyune Kim, Soo Ok
Copyright Year	2001
Abstract	An attenuated Japanese encephalitis (JE) virus SA14-14-2 (PDK) was adapted to Vero cells, a continuous cell line that has been licensed for human vaccine production, by serial passages. The resulting virus was purified by tangential flow ultrafiltration followed by sucrose density gradient ultracentrifugation, giving 2.3 mg purified virus per liter of culture supernatant. Treatment with 0.05% formalin for 4 days at 22 °C completely inactivated viral infectivity while preserving its antigenicity. The purified, inactivated JE virus was formulated with alum hydroxide and administered to mice by intraperitoneal route. In terms of its ability to induce anti-JE neutralizing antibody and to protect the immunized animal against neurovirulent virus challenge, the purified, inactivated JE virus formulated with alum was equivalent to the exiting commercial mouse brain-derived vaccine (JE-VAX, Aventis Pasteur Inc.).
Starting Page	1565
Ending Page	1573
Page Count	9
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	23
Issue Number	19
e-ISSN	15736776
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Organic Chemistry Biochemistry Microbiology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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