NDLI: Expression of glr gene encoding glutamate racemase in Bacillus licheniformis WX-02 and its regulatory effects on synthesis of poly-γ-glutamic acid

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Expression of glr gene encoding glutamate racemase in Bacillus licheniformis WX-02 and its regulatory effects on synthesis of poly-γ-glutamic acid

Content Provider	Springer Nature Link
Author	Jiang, Feng Qi, Gaofu Ji, Zhixia Zhang, Shuling Liu, Jun Ma, Xin Chen, Shouwen
Copyright Year	2011
Abstract	The glr gene, which encodes glutamate racemase involved in the conversion of l-glutamic acid to its D-isomer, was cloned and expressed in Bacillus licheniformis WX-02. Overexpression of the glr gene not only increased the production of poly-γ-glutamic acid (γ-PGA) by 22.5% but also increased the proportion of d-glutamate in γ-PGA from 77 to 85%. The activity of glutamate racemase was higher than in the original strain throughout cultivation. This is the first report that overexpression of the glr gene could enhance the l- and d-glutamate conversion in B. licheniformis WX-02 and increase the proportion of d-glutamate in γ-PGA and the yield of γ-PGA.
Starting Page	1837
Ending Page	1840
Page Count	4
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	33
Issue Number	9
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-05-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacillus licheniformis Glutamate Glutamate racemase Poly-γ-glutamic acid Biotechnology Biochemistry Applied Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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