NDLI: Single nucleotide polymorphism analysis for the production of valuable steroid intermediates in Mycobacterium neoaurum

Content Provider	Springer Nature Link
Author	Liu, Min Zhu, Zhan Tao Tao, Xin Yi Wang, Feng Qing Wei, Dong Zhi
Copyright Year	2016
Abstract	To investigate single nucleotide polymorphism (SNP) in the transformation process of phytosterol to valuable steroid intermediates in three steroid-producing Mycobacterium neoaurum strains using deep sequencing and bioinformation analysis.The assembled contig sequences from RNA sequencing of strains producing 9α-hydroxy-4-androstene-3,17-dione (9OHAD), 1,4-androstadiene-3,17-dione (ADD), and 22-hydroxy-23, 24-bisnorchola-1,4-dien-3-one (1,4-BNA) were analyzed for the presence of putative SNPs for steroid catabolism. 413, 375, and 491 SNPs were detected in the coding domain sequences and non-coding domain sequences of RNA sequencing reads of M. neoaurum strains producing 9OHAD, ADD, and BNA, respectively. Special attention was focused on SNPs associated with genes showing differential expression at proteome level, including the genes for sterol catabolism, glycerol catabolic process, signal transduction systems, transport system and energy metabolism.The work facilitates the understanding of underlying genetic changes that may be responsible for steroid accumulation in M. neoaurum and is useful for its targeted genetic engineering.
Starting Page	1881
Ending Page	1892
Page Count	12
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	11
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-08-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Deep sequencing Mycobacterium neoaurum RNA sequencing Single nucleotide polymorphism Sterol transformation Steroid Transcriptome Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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