NDLI: Carrier-free immobilized enzymes for biocatalysis

Content Provider	Springer Nature Link
Author	Roessl, Ulrich Nahálka, Jozef Nidetzky, Bernd
Copyright Year	2009
Abstract	Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assembly of the desired enzyme activity into insoluble particles in the absence of potentially denaturing chemicals required for precipitation and cross-linking. Recycling of insoluble protein particles for multiple rounds of batchwise reaction has been demonstrated in selected biotransformations. However, for application in a fully continuous biocatalytic process, low resistance to mechanical stress and high compressibility are issues for consideration on carrier-free enzyme particles.
Starting Page	341
Ending Page	350
Page Count	10
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	32
Issue Number	3
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-11-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biocatalysis Carrier-free immobilization Cross-linked enzyme aggregate Pull-down domain Self-assembling systems Biochemistry Applied Microbiology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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