NDLI: Engineered synthesis of rosmarinic acid in Escherichia coli resulting production of a new intermediate, caffeoyl-phenyllactate

Content Provider	Springer Nature Link
Author	Jiang, Jingjie Bi, Huiping Zhuang, Yibin Liu, Shaowei Liu, Tao Ma, Yanhe
Copyright Year	2015
Abstract	To achieve high production of rosmarinic acid and derivatives in Escherichia coli which are important phenolic acids found in plants, and display diverse biological activities.The synthesis of rosmarinic acid was achieved by feeding caffeic acid and constructing an artificial pathway for 3,4-dihydroxyphenyllactic acid. Genes encoding the following enzymes: rosmarinic acid synthase from Coleus blumei, 4-coumarate: CoA ligase from Arabidopsis thaliana, 4-hydroxyphenyllactate 3-hydroxylase from E. coli and d-lactate dehydrogenase from Lactobacillus pentosus, were overexpressed in an l-tyrosine over-producing E. coli strain. The yield of rosmarinic acid reached ~130 mg l−1 in the recombinant strain. In addition, a new intermediate, caffeoyl-phenyllactate (~55 mg l−1), was also produced by the engineered E. coli strain.This work not only leads to high yield production of rosmarinic acid and analogues, but also sheds new light on the construction of the pathway of rosmarinic acid in E. coli.
Starting Page	81
Ending Page	88
Page Count	8
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	38
Issue Number	1
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosynthesis Caffeoyl-phenyllactate 4-Coumarate CoA ligase Escherichia coli Rosmarinic acid Rosmarinic acid synthase Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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