NDLI: Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase

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Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase

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Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase

Content Provider	Springer Nature Link
Author	Chang, Kyung Hwa Lee, Jong Min Hwang Bo, Jeon Yoo, Ki Hyun Sohn, Bong Hee Yang, Jai Myung Chung, In Sik
Copyright Year	2007
Abstract	We examined the expression of human cyclooxygenase-1 (COX-1) in Drososphila melanogaster S2 (S2) cells transformed with cDNAs encoding β1,4-galactosyltransferase (GalT) and Galβ1,4-GlcNAc α2,6-sialyltransferase (ST). Southern blot analysis indicated that multiple copies of the glycosyltransferases genes were integrated into the S2 cell genome. A lectin blot analysis also indicated that recombinant COX-1 from S2COX-1/GalT-ST cells contained the glycan residues of β1,4-linked galactose and α2,6-linked sialic acid. The specific peroxidase activity of recombinant sialylated COX-1 from S2COX-1/GalT-ST cells was 41,250 U mg−1, indicating an increase of approximately 22% compared with a non-sialylated control (33,850 U mg−1) from S2COX-1 cells.
Starting Page	1803
Ending Page	1809
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	29
Issue Number	12
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-07-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Recombinant cyclooxygenase 1 Drosophila melanogaster S2 cells β1,4-galactosyl-transferase Galβ1,4-GlcNAc α2,6-sialyltransferase Biochemistry Applied Microbiology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase

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