NDLI: Construction of a tightly-controlled expression system for use in Pseudomonas

Content Provider	Springer Nature Link
Author	Yang, Liang Chen, Lin Shen, Lixin Duan, Kangmin
Copyright Year	2013
Abstract	Transcriptional analysis of czcCBA gene cluster in Pseudomonas aeruginosa PAO1 revealed that the promoter of this operon is tightly controlled by Zn. The promoter activity is undetectable in the uninduced condition but reaches high levels when induced. We used the czcCBA promoter to construct a tightly-controlled expression vector series, pLY vectors (pLY-A, pLY-B and pLY-C). These differed by just one base pair at the multiple cloning sites, allowing convenient in-frame cloning of any genes for expression. Using the luxCDABE reporter, this expression system was shown to be controlled by Zn in a dose-dependent manner in Pseudomonas; cloning the sacB gene encoding levansucrase onto the pLY-C vector enabled Zn-controlled growth of P. aeruginosa PAO1. These pLY vectors provide a useful tool for functional characterization of genes and controlled production of difficult or toxic proteins in Pseudomonas sp.
Starting Page	1901
Ending Page	1906
Page Count	6
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	35
Issue Number	11
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-07-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	czcCBA promoter pLY expression vectors Pseudomonas aeruginosa Zinc Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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