NDLI: Molecular Cloning and Single Nucleotide Polymorphism Detection of Buffalo DGAT1 Gene

Content Provider	Springer Nature Link
Author	Yuan, Jing Zhou, Jun Deng, Xuemei Hu, Xiaoxiang Li, Ning
Copyright Year	2007
Abstract	We report molecular cloning and single nucleotide polymorphism detection of the buffalo DGAT1 gene. Diacylglycerol acyltransferase (DGAT1) is considered the key enzyme in controlling the rate of synthesis of triglycerides. The DGAT1 gene was recently identified as a strong functional candidate gene affecting milk yield and composition in cattle. A full-length buffalo DGAT1 genomic DNA was amplified by iterative PCR based on homolog cloning. The buffalo DGAT1 gene comprises 17 exons and spans approximately 8.3 kb. The genomic structures of DGAT1 are highly conserved among mammal species. The deduced protein of buffalo DGAT1 contains 489 amino acids, showing high-sequence similarity with mammal homologs. Through PCR-SSCP analysis and sequencing, seven polymorphic positions were detected in the complete genomic region of buffalo DGAT1, and their frequencies were observed from a collection of 117 buffalo. The SNP (C/T) detected at position 11785 in exon 17 creates a substitution change for the amino acid sequence, resulting in an Ala residue (GCG) transition to a Val residue (GTG) in position 484 of buffalo DGAT1 protein. Information provided in this study will be useful in further studies to determine the role DGAT1 plays in the regulation of milk fat synthesis and quality improvement for milk in buffalo.
Starting Page	611
Ending Page	621
Page Count	11
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	45
Issue Number	7-8
e-ISSN	15734927
Language	English
Publisher	Springer US
Publisher Date	2007-06-26
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Buffalo DGAT1 Genomic structure SNP Medical Microbiology Zoology Biochemistry Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Porcine Single Nucleotide Polymorphism (SNP) Development and Population Structure of Pigs Assessed by Validated SNPs

High-Throughput Multiplex Single-Nucleotide Polymorphism (SNP) Analysis in Genes Involved in Methionine Metabolism

â€œSingle Nucleotide Polymorphism (SNP) typing of Mehsana buffalo at DGAT1 gene

Molecular Cloning, Mapping, and Polymorphism of the Porcine SCG2 gene

Cloning, Expression, and Genomic Structure of a Novel Human Rap2 Interacting Gene (RPIP9)

A TaqI PCR-RFLP Detecting a Novel SNP in Exon 2 of the Bovine POU1F1 Gene

A Novel PCR-RFLP Assay for the Detection of the Single Nucleotide Polymorphism at Position +1440 in the Human CXCR2 gene

Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed

Arg753Gln Polymorphism of the Human Toll-like Receptor-2 Gene in Children with Recurrent Febrile Infections

Molecular Cloning and Single Nucleotide Polymorphism Detection of Buffalo DGAT1 Gene

Similar Documents

Porcine Single Nucleotide Polymorphism (SNP) Development and Population Structure of Pigs Assessed by Validated SNPs

High-Throughput Multiplex Single-Nucleotide Polymorphism (SNP) Analysis in Genes Involved in Methionine Metabolism

â€œSingle Nucleotide Polymorphism (SNP) typing of Mehsana buffalo at DGAT1 gene

Molecular Cloning, Mapping, and Polymorphism of the Porcine SCG2 gene

Cloning, Expression, and Genomic Structure of a Novel Human Rap2 Interacting Gene (RPIP9)

A TaqI PCR-RFLP Detecting a Novel SNP in Exon 2 of the Bovine POU1F1 Gene

A Novel PCR-RFLP Assay for the Detection of the Single Nucleotide Polymorphism at Position +1440 in the Human CXCR2 gene

Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed

Arg753Gln Polymorphism of the Human Toll-like Receptor-2 Gene in Children with Recurrent Febrile Infections

Molecular Cloning and Single Nucleotide Polymorphism Detection of Buffalo DGAT1 Gene