NDLI: ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

Content Provider	Springer Nature Link
Author	Santos, Lucas Ferraz Oliveira, Eder Jorge Santos Silva, Aline Carvalho, Fabiana Moraes Costa, Juliana Leles Pádua, Julia Gomes
Copyright Year	2011
Abstract	Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei’s genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon–Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.
Starting Page	540
Ending Page	554
Page Count	15
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	49
Issue Number	7-8
e-ISSN	15734927
Language	English
Publisher	Springer US
Publisher Date	2011-03-22
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Passion fruit Genetic variability Molecular markers Genetic resources Biochemistry Medical Microbiology Zoology Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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