NDLI: CDNA of a Novel mRNA Expressed Predominantly in Mouse Kidney

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CDNA of a Novel mRNA Expressed Predominantly in Mouse Kidney

Content Provider	Springer Nature Link
Author	Kawamura, Takeshi Kuroda, riyuki Kimura, Yuko Lazoura, Eliada Okada, riko Okada, Hidechika
Copyright Year	2001
Abstract	We examined embryonic carcinoma (EC) cells for a potential prototype molecule of C3, the third component of complement. PCR primers, corresponding to the base sequence derived from the C3 cDNA of several species, were used for PCR amplification of the EC cell cDNA. All the PCR products obtained had the same sequence and showed no sequence homology to C3. Subsequently, cDNA clones were isolated from a mouse liver cDNA library using the PCR product as a probe. Unexpectedly, neither the base sequence of the cDNA clones nor the amino acid sequence deduced from the cDNA showed homology to C3, although partial homology was observed to a number of sequences from EST databases. We designated this new clone NCU-G1. Northern hybridization experiments revealed that NCU-G1 is expressed constitutively not only in the mouse fetus but also in various mouse tissues, and is most abundant in the kidney cortex.
Starting Page	33
Ending Page	42
Page Count	10
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	39
Issue Number	1-2
e-ISSN	15734927
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2001-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Medical Microbiology Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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