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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mitrečić, Dinko Mavrić, Sandra Branica, Božica Vrabec Gajović, Srećko |
| Copyright Year | 2008 |
| Abstract | Routine methods used to genotype mice involve isolation of DNA from partially amputated neonate’s tail, toe, or ear. The inevitable drawbacks of such techniques are the animal’s pain response and the increased time and funds required for DNA purification. In order to implement a noninvasive and simple protocol for mouse DNA isolation, we have improved the method based on samples collected by swabbing of the inner cheek. Combining alkaline and temperature lysis, it was possible to isolate a DNA solution ready for PCR in less than an hour. Testing the method on three different mouse lines showed that it is highly efficient, the volume of the PCR samples could be reduced to 25 μl, and fragments up to 800 bp were successfully amplified. This protocol reduces animal discomfort, shortens the time for DNA isolation, and enables amplification of larger DNA fragments with optimal success rate, thus considerably facilitating large-scale genotyping of different mouse lines. |
| Starting Page | 105 |
| Ending Page | 112 |
| Page Count | 8 |
| File Format | |
| ISSN | 00062928 |
| Journal | Biochemical Genetics |
| Volume Number | 46 |
| Issue Number | 3-4 |
| e-ISSN | 15734927 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2008-01-23 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | DNA isolation Buccal swabs Genotyping PCR Mouse Medical Microbiology Zoology Biochemistry Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics |
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