NDLI: PCR Amplification of a Locus with RFLP Alleles Specific to African Honey Bees

Content Provider	Springer Nature Link
Author	Hall, H. Glenn
Copyright Year	1998
Abstract	An anonymous honey bee locus, detectedpreviously with a cloned probe, has HhaI RFLP allelesspecific to African bees or common to both African andEuropean bees. To facilitate identification of thesealleles, this region, 1231, was made analyzable with thePCR. The two halves of the region, excluding thetermini, were amplified as two overlapping segments.Restriction sites were mapped, and the site differences responsible for the allelic RFLP patterns weredetermined. In the first half of the region, twopolymorphic HhaI sites are present in the commonalleles, whereas one, the other, or both of the sitesare absent in the African alleles. In the secondhalf, a third polymorphic HhaI site is present or absentin both common and African alleles. A short part of thesecond half of the region, including more of the terminus, was amplified as a third segment.Within this segment, close to this terminus, a fourthpolymorphic HhaI site is absent in some Africanalleles.
Starting Page	351
Ending Page	361
Page Count	11
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	36
Issue Number	9-10
e-ISSN	15734927
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1998-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Medical Microbiology Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mitochondrial DNA Diversity of Orchid Bee Euglossa fimbriata (Hymenoptera: Apidae) Populations Assessed by PCR-RFLP

Utility of Spermidine in PCR Amplification of Stool Samples

Melanocortin-4 Receptor Gene Polymorphisms in Obese Patients

Nuclear DNA PCR-RFLPs That Distinguish African and European Honey Bee Groups of Subspecies. II: Conversion of Long PCR Markers to Standard PCR

Expression of Vitellogenin and Transferrin in Activated Ovaries of Worker Honey Bees, Apis mellifera

PCR Error and Molecular Population Genetics

A Novel PCR-RFLP Assay for the Detection of the Single Nucleotide Polymorphism at Position +1440 in the Human CXCR2 gene

Polymorphic Analysis of the Human Phosphoglucomutase-3 Gene Based on Mismatched PCR–RFLP Technique

Nuclear DNA PCR-RFLPs That Distinguish African and European Honey Bee Groups of Subspecies. I: Comparison of Long PCR and Standard PCR to Screen for Polymorphisms

PCR Amplification of a Locus with RFLP Alleles Specific to African Honey Bees

Similar Documents

Mitochondrial DNA Diversity of Orchid Bee Euglossa fimbriata (Hymenoptera: Apidae) Populations Assessed by PCR-RFLP

Utility of Spermidine in PCR Amplification of Stool Samples

Melanocortin-4 Receptor Gene Polymorphisms in Obese Patients

Nuclear DNA PCR-RFLPs That Distinguish African and European Honey Bee Groups of Subspecies. II: Conversion of Long PCR Markers to Standard PCR

Expression of Vitellogenin and Transferrin in Activated Ovaries of Worker Honey Bees, Apis mellifera

PCR Error and Molecular Population Genetics

A Novel PCR-RFLP Assay for the Detection of the Single Nucleotide Polymorphism at Position +1440 in the Human CXCR2 gene

Polymorphic Analysis of the Human Phosphoglucomutase-3 Gene Based on Mismatched PCR–RFLP Technique

Nuclear DNA PCR-RFLPs That Distinguish African and European Honey Bee Groups of Subspecies. I: Comparison of Long PCR and Standard PCR to Screen for Polymorphisms

PCR Amplification of a Locus with RFLP Alleles Specific to African Honey Bees