NDLI: Isolation and Expression of a Novel Alligator Gene Belonging to the Sox Gene Family

Content Provider	Springer Nature Link
Author	Zheng, Jifang Hu, Nan Zhu, Muyuan Nu, Yaqing Liu, Zhen
Copyright Year	2009
Abstract	Sox genes share a highly conserved DNA-binding motif, the HMG (high mobility group)-box domain, and have diverse roles in vertebrate embryonic development. A novel SRY-related cDNA (temporarily called Sox33) isolated from the Chinese alligator (Alligator sinensis) is 1,819 bp in length, with an open reading frame from 220 to 1113 bp, encoding a protein of 298 amino acids. Two putative polyadenylation signal sequences (AATAAA) are present upstream of the poly(A) tail in the 3′ UTR (at 1255–1260 and 1774–1779). The putative protein contains an HMG-box domain most closely related to hSox12, mSox4, rtSox11, and mSox11 homologs, indicating that alligator Sox33 belongs to group C in the Sox gene family. Alligator adult and developing tissues were tested for Sox33 mRNA by independent Northern blots using a 336-bp probe (at 907–1243) between the HMG-box and the poly(A) site I and a 277-bp probe (at 1477–1754) between the two polyadenylation sites. Two transcripts (1.3 kb and 1.8 kb) in developing brain and one (1.8 kb) in adult brain were identified by the 336-bp probe; only one transcript (1.8 kb) in developing and adult brains was detected by the 277-bp probe. The results suggest that alligator Sox33 may use a different polyadenylation mechanism in the developing brain and play a role in the development and maintenance of the nervous system.
Starting Page	137
Ending Page	146
Page Count	10
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	47
Issue Number	1-2
e-ISSN	15734927
Language	English
Publisher	Springer US
Publisher Date	2009-01-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alligator (Alligator sinensis) SRY Sox gene Cloning Expression Medical Microbiology Zoology Biochemistry Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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