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| Content Provider | Springer Nature Link |
|---|---|
| Author | Orasmo, Gleice Ribeiro Oliveira Collet, Sandra A. Lapenta, Ana Sílvia Machado, Maria de Fátima P. S. |
| Copyright Year | 2007 |
| Abstract | Native polyacrylamide gel electrophoresis (PAGE) was employed to show the highest number of esterase loci and to detect α- and β-esterase polymorphisms in leaf buds of Vitis vinifera cultivars. A total of 16 esterase isozymes were detected in leaf buds from 235 plants including Italia, Rubi, Benitaka, and Brasil cultivars. Biochemical characterization of the grape esterases using ester substrates revealed α-, β-, and α/β-esterases with inhibitor tests distinguishing both carboxylesterases (EST-2, EST-3, EST-5, EST-6, EST-7, EST-8, EST-9, EST-10, and EST-16 isozymes) and acetylesterases (EST-4, EST-11, EST-12, EST-13, EST-14, EST-15 isozymes). No allele variation for α-, β-, and α/β-esterases was detected; however, EST-3 α-carboxylesterase was absent in 61.7% of vines, and EST-4 α/β-acetylesterase was absent in one vine of Rubi cv. Null EST-3 carboxylesterase phenotype (61.7%) cannot be explained in this article, but the high genetic polymorphism in four V. vinifera clones is a positive aspect for genetic selection and development of new clones with different characteristics. |
| Starting Page | 663 |
| Ending Page | 670 |
| Page Count | 8 |
| File Format | |
| ISSN | 00062928 |
| Journal | Biochemical Genetics |
| Volume Number | 45 |
| Issue Number | 9-10 |
| e-ISSN | 15734927 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2007-08-21 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Grapes Esterase Somatic mutations Polymorphism Medical Microbiology Zoology Biochemistry Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics |
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