NDLI: Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant

Content Provider	Springer Nature Link
Author	Li, Jian Gang Jiang, Zhi Qiang Xu, Liu Ping Sun, Fei Fei Guo, Jian Hua
Copyright Year	2007
Abstract	A chitinase-secreting strain CH2 was one of 353 strains isolated from rhizosphere of eggplant. Based on 16S rDNA sequence alignment and several biochemical and physiological characteristics, the strain was identified to be of Bacillus cereus. On chitin–Ayers (CA) medium, the strain secreted chitinase. Evaluation of its activity, combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), showed it to be a 15.0-KD chitinase. On glass slides, germination of the fungal spores was effectively suppressed by the bacterial suspension, supernatant from the suspension, and 0.005% solution of chitinase extracted from the strain CH2. The optimum pH for chitinase was 7.1 and optimum temperature was 40°C. At that temperature, high-level chitinolytic activity was retained for 10 days. In greenhouse experiments, suspension of the cells of the CH2 strain reduced the severity of Verticillium wilt on eggplant by 69.69%, its supernatant by 54.04%, and the enzyme diluted to 0.01% strength by 53.13% in 14 days. Strain CH2 and its chitinase have good commercial potential in controlling Verticillium wilt.
Starting Page	931
Ending Page	944
Page Count	14
File Format	PDF
ISSN	13866141
Journal	BioControl
Volume Number	53
Issue Number	6
e-ISSN	15738248
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-11-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacillus cereus Verticillium wilt Chitinase Greenhouse experiment Biocontrol Behavioural Sciences Animal Biochemistry Animal Ecology Agriculture Plant Pathology Entomology
Content Type	Text
Resource Type	Article
Subject	Insect Science Agronomy and Crop Science

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