NDLI: Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank

Content Provider	Springer Nature Link
Author	Han, T. You, C. Zhang, L. Feng, C. Zhang, C. Wang, J. Kong, F.
Copyright Year	2015
Abstract	Tobacco black shank caused by Phytophthora nicotianae is a very important oomycete disease of tobacco, and it is widely distributed around the world. In order to develop effective prevention techniques, this study examined the effects of an antagonistic bacterium, Bacillus subtilis Tpb55 strain, on the prevention of tobacco black shank in vitro and in vivo. Dual culture test results showed B. subtilis Tpb55 strain have a strong antagonism to P. nicotianae, inhibit the growth of its hyphae, and produce significant inhibition zones. Scanning electron microscopy showed that the Tpb55 strain can damage the structure of P. nicotianae hyphae, cause hyphae deformity, hyphae rupture, and protoplasm leakage. Control effects of Tpb55 strains on tobacco black shank in pot and field experiment can reach up to 70.66 and 59.34 %, respectively. In this study, Tpb55 strain was also labeled with green fluorescent protein (GFP) in order to monitor their rhizosphere colonization of tobacco. The Tpb55 strain’s colonization on tobacco roots showed a diffused distribution, largely in the root meristem and elongation zone areas. They can gather focally into microcolonies, forming a biofilm like structure. A small number of these bacteria can colonize at the intercellular space and among vascular bundles. After inoculation, Tpb55-GFP was found to colonize tobacco roots for 30 days or more. The number of bacteria peaked on the 4th day at 1.51 × 107 cfu g−1. By the 12th day, it had dropped to 1.1 × 106 cfu g−1. This study shows that the effect of Tpb55 strains on controlling of tobacco black shank is correlated to their ability to inhibit mycelia growth and ability to successfully colonize tobacco roots.
Starting Page	195
Ending Page	205
Page Count	11
File Format	PDF
ISSN	13866141
Journal	BioControl
Volume Number	61
Issue Number	2
e-ISSN	15738248
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-11-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antagonistic bacteria Colonization Tobacco black shank Bacillus subtilis Entomology Plant Pathology Agriculture Animal Ecology Animal Biochemistry Behavioral Sciences
Content Type	Text
Resource Type	Article
Subject	Insect Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana

Biocontrol of Fusarium wilt disease in muskmelon with Bacillus subtilis Y-IVI

A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris

Endophytic Bacillus subtilis WH2 containing Pinellia ternata agglutinin showed insecticidal activity against whitebacked planthopper Sogatella furcifera

Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Biological control of Radopholus similis in banana by combined application of the mutualistic endophyte Fusarium oxysporum strain 162, the egg pathogen Paecilomyces lilacinus strain 251 and the antagonistic bacteria Bacillus firmus

Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease

Effect of β-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear fruit

Biocontrol of chickpea root rot using endophytic actinobacteria

Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank

Similar Documents

Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana

Biocontrol of Fusarium wilt disease in muskmelon with Bacillus subtilis Y-IVI

A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris

Endophytic Bacillus subtilis WH2 containing Pinellia ternata agglutinin showed insecticidal activity against whitebacked planthopper Sogatella furcifera

Whole Genome Sequence of Bacillus velezensis Strain GUMT319: A Potential Biocontrol Agent Against Tobacco Black Shank Disease

Biological control of Radopholus similis in banana by combined application of the mutualistic endophyte Fusarium oxysporum strain 162, the egg pathogen Paecilomyces lilacinus strain 251 and the antagonistic bacteria Bacillus firmus

Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease

Effect of β-glucan on stress tolerances and biocontrol efficacy of Cryptococcus laurentii against Penicillium expansum in pear fruit

Biocontrol of chickpea root rot using endophytic actinobacteria

Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank