NDLI: Population Structure of in Vitro Cultured Keratinocytes

Content Provider	Springer Nature Link
Author	Vorotelyak, E. A. Chermnykh, E. S. Vasiliev, A. V. Terskikh, V. V.
Copyright Year	2005
Abstract	Analysis of behavior of isolated epidermal keratinocytes demonstrated two dominant processes within the first two days of cultivation: formation of cell aggregates and their adhesion to the substrate. Keratinocyte spreading increased in the attached aggregates, where densely packed and well spread cells could be recognized in the aggregate center and periphery, respectively. Cell spreading and proliferation was observed in the following days. Randomly distributed p63-positive cells amounted to 35% in the studied 2–3-day keratinocyte cultures. The process of epidermal cell aggregation was reproduced in the population of basal keratinocytes, where Ki-67-expressing cells amounted to about 20%. Single cells expressing keratin 19 were observed during cell aggregation in the secondary culture. We believe that the aggregation of cultured keratinocytes reflects the formation of structural-functional units (SFUs) in vivo. Disaggregated epidermal keratinocytes maintained the information relevant for self-assembly of three-dimensional structures capable of SFU formation after transplantation into the body.
Starting Page	533
Ending Page	536
Page Count	4
File Format	PDF
ISSN	10623590
Journal	Biology Bulletin of the Russian Academy of Sciences
Volume Number	32
Issue Number	6
e-ISSN	16083059
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2005-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Biochemistry Cell Biology Ecology Zoology
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences

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