NDLI: Dicarbonyl-induced accelerated aging in vitro in human skin fibroblasts

Content Provider	Springer Nature Link
Author	Sejersen, Henrik Rattan, Suresh I. S.
Copyright Year	2008
Abstract	Dicarbonyls glyoxal (GO) and methylglyoxal (MGO) produced during the autoxidation of reducing sugars are a source of macromolecular damage in cells. Since an accumulation of damaged macromolecules is a universal characteristic of aging, we have tested whether GO and MGO which cause oxidative damage to proteins and other macromolecules can bring about accelerated aging in normal human skin fibroblasts in vitro. A treatment of cells with 1.0 mM GO or 400 μM MGO leads to the appearance of senescent phenotype within 3 days, as judged by the following criteria: morphological phenotype, irreversible growth arrest and G2 arrest, increased senescence-associated β-galactosidase (SABG) activity, increased H2O2 level, increased Nξ-(carboxymethyl)-lysine (CML) protein level, and altered activities of superoxide dismutase and catalase antioxidant enzymes. This experimental model of accelerated cellular aging in vitro can be useful for studies on testing the effects of various physical, chemical and biological conditions, including natural and synthetic molecules, for the modulation of aging.
Starting Page	203
Ending Page	211
Page Count	9
File Format	PDF
ISSN	13895729
Journal	Biogerontology
Volume Number	10
Issue Number	2
e-ISSN	15736768
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-08-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oxidative stress α-oxoaldehydes Cell cycle CML-modified proteins Antioxidant enzymes Developmental Biology Geriatrics/Gerontology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Aging Gerontology Geriatrics and Gerontology

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