NDLI: Proteasome activities in the rectus abdominis muscle of young and older individuals

Content Provider	Springer Nature Link
Author	Bossola, Maurizio Pacelli, Fabio Costelli, Paola Tortorelli, Antonio Rosa, Fausto Doglietto, Giovan Battista
Copyright Year	2008
Abstract	Sarcopenia is one of the most striking effects of age, the causes and the pathogenic mechanisms being largely unknown. Unfortunately, there is limited information on the effect of aging on muscle protein breakdown in basal conditions. The present study aimed at investigating if skeletal muscle ubiquitn mRNA levels and proteasome activities vary with age in healthy individuals. Ub mRNA levels were measured by northern blot analysis whereas proteasome activities were determined by evaluating the cleavage of specific fluorogenic substrates in the rectus abdominis muscle of 14 healthy male individuals. Patients were divided in three groups according to the age: (1) 20–30 years (N = 3); (2) 31–64 years (N = 5); (3) ≥65 years (N = 6). Quantitation of the ubiquitin mRNA levels (expressed in arbitrary units) (mean (SD) showed no differences among the three groups of age (20–30 years: 1352 ± 441; 31–64 years: 1324 ± 439; ≥65 years: 884 ± 400; P = 0.33). The correlation between age and muscle ubiquitin mRNA levels was not statistically significant (r = −0.4, P = 0.26). The three proteasome activities, chymotrypsin-like (CTL), trypsin-like (TL) and peptidyl-gutamyl-peptidase (PGP), expressed as nkatal × 10−3/mg protein, were similar in the three groups of patients stratified according to the age. There was no correlation between age with either CTL (r = 0.22, P = 0.4), PGP (r = 0.002, P = 0.9), and TL (r = 0.28, P = 0.33) activities. In conclusion, the present study shows that the skeletal muscle proteasome activities do not differ with age in healthy male individuals.
Starting Page	261
Ending Page	268
Page Count	8
File Format	PDF
ISSN	13895729
Journal	Biogerontology
Volume Number	9
Issue Number	4
e-ISSN	15736768
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-03-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sarcopenia Age Skeletal muscle Ubiquitin–proteasome Activity Developmental Biology Geriatrics/Gerontology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Aging Gerontology Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

L-Lysine suppresses myofibrillar protein degradation and autophagy in skeletal muscles of senescence-accelerated mouse prone 8

Age-related loss of muscle fibres is highly variable amongst mouse skeletal muscles

Physiological and functional evaluation of healthy young and older men and women: design of the European MyoAge study

The functional consequences of age-related changes in microRNA expression in skeletal muscle

Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism

Circulating levels of adipokines and IGF-1 are associated with skeletal muscle strength of young and old healthy subjects

Metabolomic profiling reveals severe skeletal muscle group-specific perturbations of metabolism in aged FBN rats

Physical activity in older age: perspectives for healthy ageing and frailty

Frailty and muscle metabolism dysregulation in the elderly

Proteasome activities in the rectus abdominis muscle of young and older individuals

Similar Documents

L-Lysine suppresses myofibrillar protein degradation and autophagy in skeletal muscles of senescence-accelerated mouse prone 8

Age-related loss of muscle fibres is highly variable amongst mouse skeletal muscles

Physiological and functional evaluation of healthy young and older men and women: design of the European MyoAge study

The functional consequences of age-related changes in microRNA expression in skeletal muscle

Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism

Circulating levels of adipokines and IGF-1 are associated with skeletal muscle strength of young and old healthy subjects

Metabolomic profiling reveals severe skeletal muscle group-specific perturbations of metabolism in aged FBN rats

Physical activity in older age: perspectives for healthy ageing and frailty

Frailty and muscle metabolism dysregulation in the elderly

Proteasome activities in the rectus abdominis muscle of young and older individuals