NDLI: Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

Please wait, while we are loading the content...

Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

Homocysteine induces DNA damage and alterations in proliferative capacity of T-lymphocytes: a model for immunosenescence?

Association of the F352V variant of the Klotho gene with bone mineral density

Carnosine, taurine and enzyme activities of human skeletal muscle fibres from elderly subjects with osteoarthritis and young moderately active subjects

Alpha1-antitrypsin heterozygosity plays a positive role in attainment of longevity

An evolutionary heterogeneity model of late-life fecundity in Drosophila

Telomerase activity in HeLa cervical carcinoma cell line proliferation

GM-CSF activates the Jak/STAT pathway to rescue polymorphonuclear neutrophils from spontaneous apoptosis in young but not elderly individuals

PTH regulation of c-Jun terminal kinase and p38 MAPK cascades in intestinal cells from young and aged rats

Superoxide dismutase activities in long-lived Drosophila melanogaster females: chico 1 genotypes and dietary dilution

Dehydroepiandrosterone Sulphate Enhances IgG and Interferon-Gamma Production During Immunization to Tuberculosis in Young But not Aged Mice

Dietary restriction, glycolysis, hormesis and ageing

Survival and longevity improvements at extreme ages: an interpretation assuming an ecological stress theory of aging

Biogerontology : Volume 8, Issue 1, February 2007

Biogerontology : Volume 7

Biogerontology : Volume 6

Biogerontology : Volume 5

Biogerontology : Volume 4

Biogerontology : Volume 3

Biogerontology : Volume 2

Biogerontology : Volume 1

Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

Content Provider	Springer Nature Link
Author	Lacroix, Sophie Bouez, Charbel Vidal, Sandrine Cenizo, Valérie Reymermier, Corinne Justin, Virginie Vičavá, Jana Damour, Odile
Copyright Year	2006
Abstract	Cultured skin equivalent (SE, Mimeskin®) was generated by co-culturing skin fibroblasts and keratinocytes on a collagen-glycosaminoglycan-chitosan dermal substrate. In order to examine donor age effect, fibroblasts from 19- (young) or 49- (aged) year-old females were used. Culture medium was supplemented with nutrients complex containing soy extract, tomato extract, grape seed extract, white tea extract, sodium ascorbate, tocopherol acetate, zinc gluconate and BioMarine™ complex. Epidermal and dermal structure and composition were examined after 42 and 60 days of culture. In untreated samples, SE generated from young fibroblasts was superior to SE from aged fibroblasts in all characteristics. Those include number and regularity of keratinocyte layers, number of keratinocytes expressing proliferation marker Ki67, content of collagen type I, fibrillin-1, elastin, and SE lifespan. Effects of nutritional supplementation were observed in SE from both young and aged fibroblasts, however, those effects were more pronounced in SE from aged fibroblasts. In epidermis, the treatment increased number of keratinocyte layers and delayed epidermal senescence. The number of cells expressing Ki67 was nine folds higher than those of controls, and was similar to that of young cell SE. In dermis, the treatment increased mRNA synthesis of collagen I, fibrillin-1 and elastin. In conclusion, skin cell donor age had major important effect on formation of reconstructed SE. Imperfections in epidermal and dermal structure and composition as well as life span in SE from aged cells can be improved by supplementation with active nutrients.
Starting Page	97
Ending Page	109
Page Count	13
File Format	PDF
ISSN	13895729
Journal	Biogerontology
Volume Number	8
Issue Number	2
e-ISSN	15736768
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-09-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ageing Dermis Fibroblast Imedeen In vitro Mimeskin Keratinocyte Skin equivalent Developmental Biology Geriatrics/Gerontology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Aging Gerontology Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of serially passaged fibroblasts on dermal and epidermal morphogenesis in human skin equivalents

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Aging alters functionally human dermal papillary fibroblasts but not reticular fibroblasts: a new view of skin morphogenesis and aging (2008).

Biol. Proced. Online 2004;6(1): 55-60. Organotypic cocultures as skin equivalents: a complex and sophisticated in vitro system.

Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors

Lipid peroxidation-derived 4-hydroxynonenal-modified proteins accumulate in human facial skin fibroblasts during ageing in vitro

Curcumin induces stress response and hormetically modulates wound healing ability of human skin fibroblasts undergoing ageing in vitro

Repeat mild heat shock increases dermal fibroblast activity and collagen production.

Acellular dermal equivalent derived from fibroblast culture alone.

Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

Similar Documents

Effects of serially passaged fibroblasts on dermal and epidermal morphogenesis in human skin equivalents

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Aging alters functionally human dermal papillary fibroblasts but not reticular fibroblasts: a new view of skin morphogenesis and aging (2008).

Biol. Proced. Online 2004;6(1): 55-60. Organotypic cocultures as skin equivalents: a complex and sophisticated in vitro system.

Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors

Lipid peroxidation-derived 4-hydroxynonenal-modified proteins accumulate in human facial skin fibroblasts during ageing in vitro

Curcumin induces stress response and hormetically modulates wound healing ability of human skin fibroblasts undergoing ageing in vitro

Repeat mild heat shock increases dermal fibroblast activity and collagen production.

Acellular dermal equivalent derived from fibroblast culture alone.

Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors