NDLI: Olfactory Conditioning in the Third Instar Larvae of Drosophila melanogaster Using Heat Shock Reinforcement

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Olfactory Conditioning in the Third Instar Larvae of Drosophila melanogaster Using Heat Shock Reinforcement

Content Provider	Springer Nature Link
Author	Khurana, Sukant Robinson, Brooks G. Wang, Zihe Shropshire, William C. Zhong, Allen C. Garcia, Laura E. Corpuz, Jonathan Chow, Jonathan Hatch, Michael M. Precise, Eric F. Cady, Amanda Godinez, Ryan M. Pulpanyawong, Terapat Nguyen, Andrew T. Li, Wen ke Seiter, Max Jahanian, Kambiz Sun, Jeffrey C. Shah, Ruchita Rajani, Sunaina Chen, William Y. Ray, Sofia Ryazava, Natalie V. Wakou, Dorah Prabhu, Rohith K. Atkinson, Nigel S.
Copyright Year	2011
Abstract	Adult Drosophila melanogaster has long been a popular model for learning and memory studies. Now the larval stage of the fruit fly is also being used in an increasing number of classical conditioning studies. In this study, we employed heat shock as a novel negative reinforcement for larvae and obtained high learning scores following just one training trial. We demonstrated heat-shock conditioning in both reciprocal and non-reciprocal paradigms and observed that the time window of association for the odor and heat shock reinforcement is on the order of a few minutes. This is slightly wider than the time window for electroshock conditioning reported in previous studies, possibly due to lingering effects of the high temperature. To test the utility of this simplified assay for the identification of new mutations that disrupt learning, we examined flies carrying mutations in the dnc gene. While the sensitivity to heat shock, as tested by writhing, was similar for wild type and dnc homozygotes, dnc mutations strongly diminished learning. We confirmed that the learning defect in dnc flies was indeed due to mutation in the dnc gene using non-complementation analysis. Given that heat shock has not been employed as a reinforcement for larvae in the past, we explored learning as a function of heat shock intensity and found that optimal learning occurred around 41°C, with higher and lower temperatures both resulting in lower learning scores. In summary, we have developed a very simple, robust paradigm of learning in fruit fly larvae using heat shock reinforcement.
Starting Page	151
Ending Page	161
Page Count	11
File Format	PDF
ISSN	00018244
Journal	Behavior Genetics
Volume Number	42
Issue Number	1
e-ISSN	15733297
Language	English
Publisher	Springer US
Publisher Date	2011-08-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Learning Memory Fruit fly Larvae Dunce Olfaction Heat-shock Negative reinforcement Health Psychology Clinical Psychology Public Health/Gesundheitswesen
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics Genetics (clinical)

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