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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ayub, Farhana Sarker, Md. Yusuf Alam, Md. Samsul |
| Copyright Year | 2007 |
| Abstract | White spot syndrome (WSS) is considered as a great threat to commercial farming of the tiger shrimp (Penaeus monodon). The causal agent of WSS is a DNA virus called white spot syndrome virus (WSSV). The prevalence of this dreadful virus infection has been studied in five randomly selected hatcheries located in the Cox’s Bazar district of Bangladesh. Both one-step and nested polymerase chain reaction (PCR) involving two pairs of primers, namely, 146F1/146R1 and 146F2/146R2, amplifying the 1447 bp and 941 bp fragments, respectively, were conducted to detect the WSSV. Out of 60 randomly collected shrimps, 12 (20%) were found to be positive by one-step PCR, while 18 (30%) were found to be positive by nested PCR. The nested PCR was found to be much more sensitive than the one-step PCR. The shrimp specimens showing clinical signs of WSS were positive for WSSV by both one-step and nested PCR. Some of the apparently healthy samples were also found to be positive for WSSV by nested PCR. Among the two primer-pairs, the inner pair amplifying the 941 bp fragment was more sensitive than the outer primer pair amplifying the 1447 bp fragment when used in one-step PCR. |
| Starting Page | 405 |
| Ending Page | 415 |
| Page Count | 11 |
| File Format | |
| ISSN | 09676120 |
| Journal | Aquaculture International |
| Volume Number | 16 |
| Issue Number | 5 |
| e-ISSN | 1573143X |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2007-12-28 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Detection Nested PCR Penaeus monodon Prevalence White spot syndrome virus Zoology Freshwater & Marine Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Agronomy and Crop Science |
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