NDLI: Involvement of anion exchanger-2 in apoptosis of endothelial cells induced by high glucose through an mPTP-ROS-Caspase-3 dependent pathway

Content Provider	Springer Nature Link
Author	Huang, Qi Ren Li, Qing Chen, Yuan Hong Li, Li Liu, Li Li Lei, Shui Hong Chen, He Ping Peng, Wei Jie He, Ming
Copyright Year	2010
Abstract	Excess apoptosis of endothelial cells (EC) plays crucial roles in the onset and progression of vasculopathy in diabetes mellitus. Anion exchanger-2 (AE2) might be involved in the vasculopathy. However, little is known about the molecular mechanisms that AE2 mediated the apoptosis of EC. The purpose of this study was to explore the role of AE2 in the apoptosis of HUVECs induced by high glucose (HG) and its possible mechanisms. First, HUVECs were exposed to different glucose concentrations (5.5, 17.8, 35.6, 71.2 and 142.4 mmol/l, respectively, pH = 7.40) for different time points (12, 24, 48, 72, 120, and 168 h, respectively). Intracellular Cl− concentration ([Cl−]i), AE2 expression and the apoptosis were assayed. Then, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), Cl−-free media or specific RNA interference (RNAi) for AE2 was used to confirm whether AE2 could mediate the apoptosis induced by HG. Finally, the mechanisms of the AE2-mediated apoptosis were investigated by detecting mitochondrial permeability transition pore (mPTP, ΔΨm) openings, reactive oxygen species (ROS) levels and Caspase-3 activity. We found that HG upregulated the AE2 expression and activity, increased [Cl−]i and induced the apoptosis in a time- and concentration-dependent manner. The apoptosis of HUVECs by HG was possibly mediated by AE2 through an mPTP-ROS-Caspase-3 dependent pathway. These findings suggested that AE2 was likely to be a glucose-sensitive transmembrane transporter and a novel potential therapeutic target for diabetic vasculopathy.
Starting Page	693
Ending Page	704
Page Count	12
File Format	PDF
ISSN	13608185
Journal	Apoptosis
Volume Number	15
Issue Number	6
e-ISSN	1573675X
Language	English
Publisher	Springer US
Publisher Date	2010-02-24
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anion exchanger-2 Apoptosis Caspase-3 Endothelial cells Mitochondrial permeability transition pore Reactive oxygen species Virology Biochemistry Oncology Cell Biology Cancer Research
Content Type	Text
Resource Type	Article
Subject	Pharmaceutical Science Biochemistry (medical) Cell Biology Clinical Biochemistry Cancer Research Pharmacology

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