NDLI: Swelling rather than shrinkage precedes apoptosis in serum-deprived vascular smooth muscle cells

Content Provider	Springer Nature Link
Author	Platova, Alexandra Koltsova, Svetlana V. Hamet, Pavel Grygorczyk, Ryszard Orlov, Sergei N.
Copyright Year	2012
Abstract	Contrasting cell volume behaviours (swelling vs. shrinkage) are considered as criteria to distinguish necrosis from apoptosis. In this study, we employed a time-lapse, dual-image surface reconstruction technique to assess the volume of single vascular smooth muscle cells transfected with E1A-adenoviral protein (E1A-VSMC) and undergoing rapid apoptosis in the absence of growth factors or in the presence of staurosporine. After 30- to 60-min lag-phase, serum-deprived E1A-VSMC volume was increased by ~40%, which preceded maximal increments of caspase-3 activity and chromatin cleavage. Swollen cells underwent rapid apoptotic collapse, documented by plasma membrane budding, and terminated in 10–15 min by the formation of numerous apoptotic bodies. Suppression of apoptosis by inhibition of Na+,K+-ATPase and activation of cAMP signalling with ouabain and forskolin, respectively, completely abolished the swelling of serum-deprived E1A-VSMC. In contrast to serum deprivation, apoptotic collapse of staurosporine-treated E1A-VSMC preceded attenuation of their volume by ~30%. Neither transient hyposmotic swelling nor isosmtotic shrinkage triggered apoptosis. Our results show that cell shrinkage can not be considered as ubiquitous hallmark of apoptosis. The involvement of stimulus-specific cell volume perturbations in initiation and progression of apoptosis in vascular smooth muscle cells should be examined further.
Starting Page	429
Ending Page	438
Page Count	10
File Format	PDF
ISSN	13608185
Journal	Apoptosis
Volume Number	17
Issue Number	5
e-ISSN	1573675X
Language	English
Publisher	Springer US
Publisher Date	2012-01-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vascular smooth muscle Apoptosis Na+,K+-ATPase cAMP Cell volume Cell Biology Virology Cancer Research Biochemistry Oncology
Content Type	Text
Resource Type	Article
Subject	Pharmaceutical Science Biochemistry (medical) Cell Biology Clinical Biochemistry Cancer Research Pharmacology

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