NDLI: Hydrogen as an energy source for the human pathogen Bilophila wadsworthia

Content Provider	Springer Nature Link
Author	Silva, Sofia M. Venceslau, Sofia S. Fernandes, Cláudia L. V. Valente, Filipa M. A. Pereira, Inês A. C.
Copyright Year	2007
Abstract	The gram-negative anaerobic gut bacterium Bilophila wadsworthia is the third most common isolate in perforated and gangrenous appendicitis, being also found in a variety of other infections. This organism performs a unique kind of anaerobic respiration in which taurine, a major organic solute in mammals, is used as a source of sulphite that serves as terminal acceptor for the electron transport chain. We show here that molecular hydrogen, one of the major products of fermentative bacteria in the colon, is an excellent growth substrate for B. wadsworthia. We have quantified the enzymatic activities associated with the oxidation of H2, formate and pyruvate for cells obtained in different growth conditions. The cell extracts present high levels of hydrogenase activity, and up to five different hydrogenases can be expressed by this organism. One of the hydrogenases appears to be constitutive, whereas the others show differential expression in different growth conditions. Two of the hydrogenases are soluble and are recognised by antibodies against a [FeFe] hydrogenase of a sulphate reducing bacterium. One of these hydrogenases is specifically induced during fermentative growth on pyruvate. Another two hydrogenases are membrane-bound and show increased expression in cells grown with hydrogen. Further work should be carried out to reveal whether oxidation of hydrogen contributes to the virulence of B. wadsworthia.
Starting Page	381
Ending Page	390
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	93
Issue Number	4
e-ISSN	15729699
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-12-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bilophila wadsworthia Hydrogen Hydrogenase Formate dehydrogenase Gut bacteria Soil Science & Conservation Plant Sciences Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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