NDLI: Abundances, diversity and seasonality of (non-extremophilic) Archaea in Alpine freshwaters

Content Provider	Springer Nature Link
Author	Reitschuler, Christoph Hofmann, Katrin Illmer, Paul
Copyright Year	2016
Abstract	The objectives of this study were to assess abundances and community compositions of Archaea within a heterogeneous set of freshwater systems in the Austrian Alps. Seasonal changes and geographical differences within Archaea, considering abiotic and biotic factors (e.g. temperature, pH, total organic carbon (TOC), NH4 +, bacteria, fungi), were analysed in this context. Water samples were collected from 8 lakes, 10 creeks and the river Inn in 2014. Qualitative-quantitative data were derived via a comprehensive set of (quantitative) PCR assays and PCR-DGGE (denaturing gradient gel electrophoresis) based methodology, which was evaluated concerning specificity and reliability either previously or in this study. QPCR-derived archaeal abundances reached values of 103 copies mL−1 on average, with a peak in winter-spring (‘Cold Peak’), and covered 0–15 % (average: 1 %) of the microbial populations. This peak correlated with significantly raised TOC and low NH4 + levels during the cold seasons. Stagnant waters showed significantly higher archaeal abundances and diversities than flowing ones. Among methanogens, Methanosarcinales were the most common order. PCR-DGGE data showed that the archaeal communities were site-specific and could function as an ecological marker, in contrast to the more heterogeneous and unsteady bacterial and fungal community. This is attributable to the highly heterogeneous community of methanogenic Archaea (MA, Euryarchaeota), while only two species, Nitrosopumilus maritimus and Ca. Nitrososphaera gargensis, were found to be the ubiquitous representatives of ammonia-oxidizing Archaea (AOA, Thaumarchaeota) in Alpine freshwaters. This work emphasises the diversity, distribution and seasonality of non-extremophilic Archaea in Alpine freshwaters, with a first insight into their ecophysiological potential.
Starting Page	855
Ending Page	868
Page Count	14
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	109
Issue Number	6
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-03-22
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alpine freshwater Methanogens Ammonia-oxidizing Archaea Non-extremophilic Archaea Archaeal seasonality Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Abundances, diversity and seasonality of (non-extremophilic) Archaea in Alpine freshwaters

An integrated study reveals diverse methanogens, Thaumarchaeota, and yet-uncultivated archaeal lineages in Armenian hot springs

Archaeal Distribution in Moonmilk Deposits from Alpine Caves and Their Ecophysiological Potential

Spatial distribution and abundance of ammonia-oxidizing microorganisms in deep-sea sediments of the Pacific Ocean

Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China

Temporal patterns of prokaryotic abundance, community structure and microbial activity in glacier foreland soils

Drivers of archaeal ammonia-oxidizing communities in soil

Comparative analysis of archaeal 16S rRNA and amoA genes to estimate the abundance and diversity of ammonia-oxidizing archaea in marine sediments

Abundances, diversity and seasonality of (non-extremophilic) Archaea in Alpine freshwaters

Similar Documents

Abundances, diversity and seasonality of (non-extremophilic) Archaea in Alpine freshwaters

An integrated study reveals diverse methanogens, Thaumarchaeota, and yet-uncultivated archaeal lineages in Armenian hot springs

Archaeal Distribution in Moonmilk Deposits from Alpine Caves and Their Ecophysiological Potential

Spatial distribution and abundance of ammonia-oxidizing microorganisms in deep-sea sediments of the Pacific Ocean

Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China

Temporal patterns of prokaryotic abundance, community structure and microbial activity in glacier foreland soils

Drivers of archaeal ammonia-oxidizing communities in soil

Comparative analysis of archaeal 16S rRNA and amoA genes to estimate the abundance and diversity of ammonia-oxidizing archaea in marine sediments

Abundances, diversity and seasonality of (non-extremophilic) Archaea in Alpine freshwaters