NDLI: Regulation of pssA and pssB gene expression in Rhizobium leguminosarum bv. trifolii in response to environmental factors

Content Provider	Springer Nature Link
Author	Janczarek, Monika Skorupska, Anna
Copyright Year	2004
Abstract	Exopolysaccharide (EPS) produced by Rhizobium leguminosarum plays an important role in indeterminate-type nodulation. The pssA gene, encoding the first glycosyl transferase and pssB, encoding inositol monophosphatase, are involved in EPS synthesis and regulation in R. leguminosarum bv. trifolii. Using pssA-lacZ and pssB-lacZ plasmid-borne transcriptional fusions, a stimulating effect of phosphate on the transcription of both genes was observed. The increase in the transcription of pssA and pssB genes was correlated with a significant enhancement in EPS production in rhizobia grown in the high phosphate concentrations. In the presence of ammonium, transcription of the pss-lacZ fusions was repressed, especially the pssB-lacZ fusion, but EPS production still remained enhanced. Root exudate positively influenced the level of pss-lacZ transcription. These results indicate a complex regulation of pss gene transcription by several environmental factors. In addition, the expression of pssA and pssB genes in the symbiotic state was examined using pss-gusA plasmid-borne transcriptional fusions. It was found that the pssA-gusA fusion was not expressed in the nodules. The pssB-gusA fusion was expressed mainly in the infection zone and interzone of the nodule and the pattern of expression was dependent on the plant phosphate availability.
Starting Page	217
Ending Page	227
Page Count	11
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	85
Issue Number	3
e-ISSN	15729699
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Food Science Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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