NDLI: Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum

Content Provider	Springer Nature Link
Author	Gemiarto, Adrian Tandhyka Ninyio, Nathaniel Nyakaat Lee, Siew Wei Logis, Joko Fatima, Ayesha Chan, Eric Wei Chiang Lim, Crystale Siew Ying
Copyright Year	2015
Abstract	The emergence of antibiotic-resistant bacterial pathogens, especially Gram-negative bacteria, has driven investigations into suppressing bacterial virulence via quorum sensing (QS) inhibition strategies instead of bactericidal and bacteriostatic approaches. Here, we investigated several bee products for potential compound(s) that exhibit significant QS inhibitory (QSI) properties at the phenotypic and molecular levels in Chromobacterium violaceum ATCC 12472 as a model organism. Manuka propolis produced the strongest violacein inhibition on C. violaceum lawn agar, while bee pollen had no detectable QSI activity and honey had bactericidal activity. Fractionated manuka propolis (pooled fraction 5 or PF5) exhibited the largest violacein inhibition zone (24.5 ± 2.5 mm) at 1 mg dry weight per disc. In C. violaceum liquid cultures, at least 450 µg/ml of manuka propolis PF5 completely inhibited violacein production. Gene expression studies of the vioABCDE operon, involved in violacein biosynthesis, showed significant (≥two-fold) down-regulation of vioA, vioD and vioE in response to manuka propolis PF5. A potential QSI compound identified in manuka propolis PF5 is a hydroxycinnamic acid-derivative, isoprenyl caffeate, with a [M−H] of 247. Complete violacein inhibition in C. violaceum liquid cultures was achieved with at least 50 µg/ml of commercial isoprenyl caffeate. In silico docking experiments suggest that isoprenyl caffeate may act as an inhibitor of the violacein biosynthetic pathway by acting as a competitor for the FAD-binding pockets of VioD and VioA. Further studies on these compounds are warranted toward the development of anti-pathogenic drugs as adjuvants to conventional antibiotic treatments, especially in antibiotic-resistant bacterial infections.
Starting Page	491
Ending Page	504
Page Count	14
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	108
Issue Number	2
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-06-10
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Quorum sensing inhibition Manuka propolis Isoprenyl caffeate vio operon Antibiotic resistance Chromobacterium violaceum Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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