NDLI: Statistical differences between relative quantitative molecular fingerprints from microbial communities

Content Provider	Springer Nature Link
Author	Portillo, M. C. Gonzalez, J. M.
Copyright Year	2008
Abstract	Molecular fingerprints of microbial communities are a common method for the analysis and comparison of environmental samples. The significance of differences between microbial community fingerprints was analyzed considering the presence of different phylotypes and their relative abundance. A method is proposed by simulating coverage of the analyzed communities as a function of sampling size applying a Cramér-von Mises statistic. Comparisons were performed by a Monte Carlo testing procedure. As an example, this procedure was used to compare several sediment samples from freshwater ponds using a relative quantitative PCR-DGGE profiling technique. The method was able to discriminate among different samples based on their molecular fingerprints, and confirmed the lack of differences between aliquots from a single sample.
Starting Page	157
Ending Page	163
Page Count	7
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	94
Issue Number	2
e-ISSN	15729699
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-01-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbial community Fingerprinting Statistical comparison PCR-DGGE Soil Science & Conservation Plant Sciences Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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Statistical differences between relative quantitative molecular fingerprints from microbial communities