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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chen, Zhihuan Liu, Pengyan Liu, Yanhua Tang, Hui Chen, Yunping Zhang, Liping |
| Copyright Year | 2014 |
| Abstract | Triacylglycerols (TAGs), synthesized in the microsomal membranes of eukaryotes, serve as a primary storage form of carbon and energy in microorganisms. For this reason, TAGs produced by organisms have great potential to become biofuels and facilitate researchers to look for alternative renewable sources of energy. The present study describes the identification and functional characterization of a type-2 diacylglycerol acyltransferase from Rhodosporidium diobovatum, designated as RdDGAT, which catalyzed the final step of TAG synthesis. A full-length cDNA clone for RdDGAT was obtained, and its biological activity was proven by being expressed in a Saccharomyces cerevisiae quadruple mutant that was defective in TAG synthesis. Enzymatic assays were performed and finally the existence of TAGs in the transformed Saccharomyces cerevisiae quadruple mutant was determined using the method of thin-layer chromatography. Substrate preference experiments revealed that RdDGAT preferred unsaturated fatty acids over saturated ones. Through further analysis, we assume that the evolution and expression characteristics of the RdDGAT gene perhaps is the result of adaption to its oligotrophic and cold living environment. |
| Starting Page | 1127 |
| Ending Page | 1137 |
| Page Count | 11 |
| File Format | |
| ISSN | 00036072 |
| Journal | Antonie van Leeuwenhoek |
| Volume Number | 106 |
| Issue Number | 6 |
| e-ISSN | 15729699 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2014-10-08 |
| Publisher Place | Cham |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Triacylglycerols Diacylglycerol acyltransferase Rhodosporidium diobovatum Heterologous expression Substrate preference Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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