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| Content Provider | Springer Nature Link |
|---|---|
| Author | Aliliche, Khadidja Beghalem, Hamida Landoulsi, Ahmed Chriki, Ali |
| Copyright Year | 2016 |
| Abstract | Isolates from root nodules of Hedysarum flexuosum, sampled from north region of Algeria, were analyzed on the basis of their phenotypic and molecular characteristics. They were tested for their tolerance to NaCl, pH, temperatures, antibiotics and heavy metals resistance. Interestingly, the isolate Hf_04N appeared resistant to ZnCl2 (50 μg/mL) and grew at high saline concentration up to 9 %. The phylogenetic positions of five isolates were studied by comparative sequence analysis of 16S rRNA, recA, nifH and nodD genes. There were grouped close to the Rhizobium sullae type strain in relation to their 16S rRNA, recA and nifH genes-based phylogenies. By contrast, the tree of nodD gene was not congruent with ribosomal, housekeeping and nitrogen fixation genes. We suggest that our strains have a novel nodD gene. The detection of conserved domains of NodD protein and nitrogenase reductase enzyme, confirm their ability to nodulate and fix nitrogen. |
| Starting Page | 897 |
| Ending Page | 906 |
| Page Count | 10 |
| File Format | |
| ISSN | 00036072 |
| Journal | Antonie van Leeuwenhoek |
| Volume Number | 109 |
| Issue Number | 7 |
| e-ISSN | 15729699 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2016-03-31 |
| Publisher Place | Cham |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Hedysarum flexuosum nifH nodD Rhizobium sullae recA 16S rRNA Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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