NDLI: Comparative biogeography of Chromobacterium from the neotropics

Content Provider	Springer Nature Link
Author	Lima Bittencourt, Cláudia I. Costa, Patrícia S. Hollatz, Claudia Raposeiras, Ruy Santos, Fabrício R. Chartone Souza, Edmar Nascimento, Andréa M. A.
Copyright Year	2010
Abstract	The genus Chromobacterium encompasses free-living Gram-negative bacteria. Until 2007, the genus consisted of only one species but six species are now recognized. Chromobacterium violaceum is the type species of the genus and is commonly found in soil and water in tropical and sub-tropical regions. We have investigated a collection of 111 isolates displaying violet pigmentation from undisturbed aquatic and soil environments from Brazilian Cerrado ecosystem. The 16S rRNA gene phylogeny revealed that all isolates were allocated in a monophyletic cluster inside the Chromobacterium genus and formed few clusters related most closely with Chromobacterium piscinae. The two sets of isolates from water and soil were analyzed by the repetitive extragenic palindromic (rep)-PCR genomic fingerprinting technique using a BOX-AR1 primer. The antimicrobial susceptibility and the different carbon sources utilized by these isolates were also investigated. Physiological profiles of the isolates generated by BIOLOG GN2 plates showed great versatility in the substrate utilization, much higher than the C. violaceum ATCC 12472. All isolates exhibited a high minimum inhibitory concentration (MIC) to ampicillin (MIC > 512 μg/ml) and were inhibited by ciprofloxacin, tetracycline and mercury at the lowest concentration tested (MIC < 2 μg/ml). Thirteen BOX-PCR band patterns were identified from 33 individual fingerprints. Eleven patterns provided evidence for endemic distributions. Antimicrobial susceptibility and BOX-PCR fingerprint clustering showed a clear distinction between Chromobacterium isolates from the water and soil. The results suggested that microenvironment barriers such as water and soil can play an important role in the periodic selection and diversification of Chromobacterium population ecotypes.
Starting Page	355
Ending Page	370
Page Count	16
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	99
Issue Number	2
e-ISSN	15729699
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-08-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chromobacterium 16S rDNA BOX-PCR Antimicrobial susceptibility BIOLOG Cerrado Neotropical Medical Microbiology Soil Science & Conservation Plant Sciences Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods

Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis

Novel actinobacteria from marine sponges

Assessment of the genetic diversity of Xylella fastidiosa isolated from citrus in Brazil by PCR-RFLP of the 16S rDNA and 16S-23S intergenic spacer and rep-PCR fingerprinting

Isolation and characterization of alachlor-degrading actinomycetes from soil

16S rDNA library-based analysis of ruminal bacterial diversity

BOX-PCR is an Adequate Tool for Typing Aeromonas spp.

A comparative study on the phylogenetic diversity of culturable actinobacteria isolated from five marine sponge species

Streptomyces roseoalbus sp. nov., an actinomycete isolated from soil in Yunnan, China

Comparative biogeography of Chromobacterium from the neotropics

Similar Documents

Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods

Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis

Novel actinobacteria from marine sponges

Assessment of the genetic diversity of Xylella fastidiosa isolated from citrus in Brazil by PCR-RFLP of the 16S rDNA and 16S-23S intergenic spacer and rep-PCR fingerprinting

Isolation and characterization of alachlor-degrading actinomycetes from soil

16S rDNA library-based analysis of ruminal bacterial diversity

BOX-PCR is an Adequate Tool for Typing Aeromonas spp.

A comparative study on the phylogenetic diversity of culturable actinobacteria isolated from five marine sponge species

Streptomyces roseoalbus sp. nov., an actinomycete isolated from soil in Yunnan, China

Comparative biogeography of Chromobacterium from the neotropics