NDLI: Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.

Content Provider	Springer Nature Link
Author	Jiang, Shumei Sun, Wei Chen, Minjie Dai, Shikun Zhang, Long Liu, Yonghong Lee, Kyung Jin Li, Xiang
Copyright Year	2007
Abstract	This study describes actinobacteria isolated from the marine sponge Haliclona sp. collected in shallow water of the South China Sea. A total of 54 actinobacteria were isolated using media selective for actinobacteria. Species diversity and natural product diversity of isolates from marine sponge Haliclona sp. were analysed. Twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis. The 16S rRNA genes of 24 isolates were digested by restriction enzymes TaqI and MspI and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Streptomyces, Nocardiopsis, Micromonospora and Verrucosispora; one other isolate was recovered that does not belong to known genera based on its unique 16S rRNA gene sequence. To our knowledge, this is the first report of a bacterium classified as Verrucosispora sp. that has been isolated from a marine sponge. The majority of the strains tested belong to the genus Streptomyces and three isolates may be new species. All of the 24 isolates were screened for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). PKS and NRPS sequences were detected in more than half of the isolates and the different “PKS-I—PKS-II—NRPS” combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution.
Starting Page	405
Ending Page	416
Page Count	12
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	92
Issue Number	4
e-ISSN	15729699
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-06-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Marine sponge Actinobacteria 16S rRNA RFLP PKS NRPS Soil Science & Conservation Plant Sciences Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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