NDLI: Adhesion and cytotoxicity of Aeromonas caviae to rabbit intestinal epithelium ex vivo

Content Provider	Springer Nature Link
Author	Santos, Paula Azevedo Pereira, Ana Claudia Machado Braga, Ricardo Luís Lopes Rosa, Ana Cláudia Paula Freitas Almeida, Angela Corrêa
Copyright Year	2016
Abstract	Aeromonads are considered potential pathogens for humans and animals and are responsible for the etiology of intestinal and extraintestinal diseases. The presence of Aeromonas spp. in food and water shows that it is an important vehicle of infection in humans. The pathology caused by these bacteria involves several virulence factors, such as the ability to produce toxins, adhesion and invasion. The present study investigated the interaction of five Aeromonas caviae strains isolated from human diarrheic faeces with rabbit ileal and colonic mucosa ex vivo, using in vitro organ culture model. The in vitro adhesion assays using cultured tissue were performed with A. caviae strains co-incubated with intestinal fragments of ileum and colon over a period of 6 h. The fragments were analyzed by light and electron microscopy. All strains adhered to rabbit ileal and colonic mucosa ex vivo, with higher degree of adherence presented on colonic mucosa. The typical aggregative adherence pattern was observed among strains studied. Through electron and light microscopy, we observed extensive colonization of ileal and colonic mucosa, large mucus production, biofilm formation and morphological alterations such as intense vacuolization, structural disorganization, cell extrusion and destruction of the villi. These results demonstrate that in vitro organ culture of intestinal mucosa from rabbit may be used to investigate Aeromonas spp. pathogenesis. Finally, our results support the pathogenic potential of Aeromonas emphasising their importance in public health.
Starting Page	1261
Ending Page	1270
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	109
Issue Number	9
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-07-09
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aeromonas caviae In vitro organ culture (IVOC) Rabbit Adhesion Cytotoxicity Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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