NDLI: APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity

Content Provider	Springer Nature Link
Author	Kaur, Parvinder Lingner, Anja Singh, Bijinder Böer, Erik Polajeva, Jelena Steinborn, Gerhard Bode, Rüdiger Gellissen, Gerd Satyanarayana, Tulasi Kunze, Gotthard
Copyright Year	2006
Abstract	The extracellular acid phosphatase-encoding Arxula adeninivorans APHO1 gene was isolated using degenerated specific oligonucleotide primers in a PCR screening approach. The gene harbours an ORF of 1449 bp encoding a protein of 483 amino acids with a calculated molecular mass of 52.4 kDa. The sequence includes an N-terminal secretion sequence of 17 amino acids. The deduced amino acid sequence exhibits 54% identity to phytases from Aspergillus awamori, Asp. niger and Asp. ficuum and a more distant relationship to phytases of the yeasts Candida albicans and Debaryomyces hansenii (36–39% identity). The sequence contains the phosphohistidine signature and the conserved active site sequence of acid phosphatases. APHO1 expression is induced under conditions of phosphate limitation. Enzyme isolates from wild and recombinant strains with the APHO1 gene expressed under control of the strong A. adeninivorans-derived TEF1 promoter were characterized. For both proteins, a molecular mass of approx. 350 kDa, corresponding to a hexameric structure, a pH optimum of pH 4.8 and a temperature optimum of 60°C were determined. The preferred substrates include p-nitrophenyl-phosphate, pyridoxal-5-phosphate, 3-indoxyl-phosphate, 1-naphthylphosphate, ADP, glucose-6-phosphate, sodium-pyrophosphate, and phytic acid. Thus the enzyme is a secretory acid phosphatase with phytase activity and not a phytase as suggested by strong homology to such enzymes.
Starting Page	45
Ending Page	55
Page Count	11
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	91
Issue Number	1
e-ISSN	15729699
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-10-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arxula adeninivorans APHO1 Secretory acid phosphatase Phytase Yeast Soil Science & Conservation Plant Sciences Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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