NDLI: Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine

Content Provider	Springer Nature Link
Author	Piri, Giorgia Zara, Severi Pinna, Giovanni Antonio Farris, Giovanni Budroni, Marilena
Copyright Year	2004
Abstract	Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed. We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in the Y region of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.
Starting Page	29
Ending Page	36
Page Count	8
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	85
Issue Number	1
e-ISSN	15729699
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Food Science Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine