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| Content Provider | Springer Nature Link |
|---|---|
| Author | Madhaiyan, Munusamy Poonguzhali, Selvaraj Lee, Jung Sook Lee, Keun Chul Hari, Kuppusamy |
| Copyright Year | 2011 |
| Abstract | A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716T. However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major components of the fatty acid profile are iso-C15:0, anteiso-C15:0, iso-C17:0 and anteiso-C17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012T (=DSM 21911T = NCCB 100267T). |
| Starting Page | 437 |
| Ending Page | 444 |
| Page Count | 8 |
| File Format | |
| ISSN | 00036072 |
| Journal | Antonie van Leeuwenhoek |
| Volume Number | 100 |
| Issue Number | 3 |
| e-ISSN | 15729699 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2011-06-14 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacillus rhizosphaerae sp. nov. 16S rRNA N-fixation Soil Science & Conservation Plant Sciences Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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