NDLI: Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes

Content Provider	Springer Nature Link
Author	Khan, Md Imran Anis, Mohammad
Copyright Year	2012
Abstract	A comparative performance of two different media formulations (woody plant medium (WPM) and Murashige and Skoog’s (MS) medium) for their ability to inflict in vitro shoot development in nodal segments of Salix tetrasperma Roxb. has been carried out. Thidiazuron (TDZ) in various concentrations was used as a supplement to the basal media. Media types, TDZ concentrations, exposure duration and culture regimes played an important role in affecting multiple shoot production. WPM supplemented with 2.5 μM TDZ for 4 weeks exposure was found to be the best for maximum (4.53 ± 0.27) shoots production in vitro. Transfer to a secondary medium consisting of 6-benzyladenine (1.0 μM) and α-naphthalene acetic acid (0.5 μM) enhanced the multiplication rate and by the end of 12 weeks, 20.33 ± 0.33 shoots with shoot length, 4.70 ± 0.26 cm were produced on WPM. Rooting of the regenerated shoots was achieved on half strength basal media (either WPM or MS) containing 0.5 μM indole-3-butyric acid. In all the experiments, different growth parameters were scored and WPM was found to be superior to MS medium. The regenerated plantlets were successfully acclimatized in the field with about 81 % survival.
Starting Page	95
Ending Page	103
Page Count	9
File Format	PDF
ISSN	01674366
Journal	Agroforestry Systems
Volume Number	86
Issue Number	1
e-ISSN	15729680
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-03-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thidiazuron Salix tetrasperma Roxb. Media types Exposure duration Growth parameters Phytoremediation Conservation Forestry Agriculture
Content Type	Text
Resource Type	Article
Subject	Forestry Agronomy and Crop Science

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