NDLI: Three-Dimensional Modeling and Quantitative Analysis of Gap Junction Distributions in Cardiac Tissue

Content Provider	Springer Nature Link
Author	Lackey, Daniel P. Carruth, Eric D. Lasher, Richard A. Boenisch, Jan Sachse, Frank B. Hitchcock, Robert W.
Copyright Year	2011
Abstract	Gap junctions play a fundamental role in intercellular communication in cardiac tissue. Various types of heart disease including hypertrophy and ischemia are associated with alterations of the spatial arrangement of gap junctions. Previous studies applied two-dimensional optical and electron-microscopy to visualize gap junction arrangements. In normal cardiomyocytes, gap junctions were primarily found at cell ends, but can be found also in more central regions. In this study, we extended these approaches toward three-dimensional reconstruction of gap junction distributions based on high-resolution scanning confocal microscopy and image processing. We developed methods for quantitative characterization of gap junction distributions based on analysis of intensity profiles along the principal axes of myocytes. The analyses characterized gap junction polarization at cell ends and higher-order statistical image moments of intensity profiles. The methodology was tested in rat ventricular myocardium. Our analysis yielded novel quantitative data on gap junction distributions. In particular, the analysis demonstrated that the distributions exhibit significant variability with respect to polarization, skewness, and kurtosis. We suggest that this methodology provides a quantitative alternative to current approaches based on visual inspection, with applications in particular in characterization of engineered and diseased myocardium. Furthermore, we propose that these data provide improved input for computational modeling of cardiac conduction.
Starting Page	2683
Ending Page	2694
Page Count	12
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	39
Issue Number	11
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2011-08-06
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tissue modeling Cardiac tissue Confocal microscopy Biomedical image processing Connexin 43 Mechanics Biophysics and Biological Physics Biomedicine general Biochemistry Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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