NDLI: Birefringence and Second Harmonic Generation on Tendon Collagen Following Red Linearly Polarized Laser Irradiation

Content Provider	Springer Nature Link
Author	Silva, Daniela Fátima Teixeira Gomes, Anderson Stevens Leonidas Campos Vidal, Benedicto Ribeiro, Martha Simões
Copyright Year	2012
Abstract	Regarding the importance of type I collagen in understanding the mechanical properties of a range of tissues, there is still a gap in our knowledge of how proteins perform such work. There is consensus in literature that the mechanical characteristics of a tissue are primarily determined by the organization of its molecules. The purpose of this study was to characterize the organization of non-irradiated and irradiated type I collagen. Irradiation was performed with a linearly polarized HeNe laser (λ = 632.8 nm) and characterization was undertaken using polarized light microscopy to investigate the birefringence and second harmonic generation to analyze nonlinear susceptibility. Rats received laser irradiation (P = 6.0 mW, I = 21.2 mW/cm2, E ≈ 0.3 J, ED = 1.0 J/cm2) on their healthy Achilles tendons, which after were extracted to prepare the specimens. Our results show that irradiated samples present higher birefringence and greater non-linear susceptibility than non-irradiated samples. Under studied conditions, we propose that a red laser with polarization direction aligned in parallel to the tendon long axis promotes further alignment on the ordered healthy collagen fibrils towards the electric field incident. Thus, prospects for biomedical applications for laser polarized radiation on type I collagen are encouraging since it supports greater tissue organization.
Starting Page	752
Ending Page	762
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	41
Issue Number	4
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2012-12-18
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Animal model Form birefringence He–Ne laser Intrinsic birefringence Nonlinear susceptibility Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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